ForestGEN
Forest EST and Genome database

Description

We developed a comprehensive DNA barcode library of the Japanese woody flora representing 43 orders, 99 families, 303 genera and 834 species and comprising 77.3% of genera and 72.2% of species of woody plants in Japan. A total of 6,216 plant specimens were collected from 223 sites across the subtropical, temperate, boreal and alpine biomes in Japan with most species represented by multiple accessions. This library utilized three chloroplast DNA regions (rbcL, trnH-psbA and matK) and consists of 14,404 barcode sequences.

Suzuki Setsuko, Kensuke Yoshimura, Saneyoshi Ueno, James Raymond Peter Worth, Tokuko Ujino-Ihara, Hiroshi Yoshimaru
Forestry and Forest Products Research Institute (FFPRI)
Department of Forest Molecular Genetics and Biotechnology

Toshio Katsuki
Forestry and Forest Products Research Institute (FFPRI)
Tama Forest Science Garden

Shuichi Noshiro, Tomoyuki Fujii
Forestry and Forest Products Research Institute (FFPRI)
Department of Wood Properties and Processing

Takahisa Arai
Tohoku University
Tohoku University Botanical Gardens

Number of sequence : 3193 (matK), 1000 (rbcL), and 2000 (trnH-psbA)

# Acknowledgements #

This DNA barcode database for the woody plants of Japan was supported by a Grant-in-Aid for Scientific Research (20248017, 25292098) from the Japan Society for the Promotion of Science, and the support program of FFPRI for researchers having family responsibilities.

# References #

1. Suzuki Setsuko, Kensuke Yoshimura, Saneyoshi Ueno, James Raymond Peter Worth, Tokuko Ujino-Ihara, Toshio Katsuki, Shuichi Noshiro, Tomoyuki Fujii, Takahisa Arai, Hiroshi Yoshimaru (2021). A DNA barcode database for the woody plants of Japan. bioRxiv doi:10.1101/2021.11.11.468310

2. Setsuko S, Yoshimura K, Ueno S, Worth JRP, Ujino-Ihara T, Katsuki T, Noshiro S, Fujii T, Arai T, Yoshimaru H. A DNA barcode reference library for the native woody seed plants of Japan. Mol Ecol Resour. 2023 May;23(4):855-871. doi: 10.1111/1755-0998.13748. Epub 2023 Jan 24. PMID: 36694075

# Link #

Department of Forest Molecular Genetics and Biotechnology
Tama Forest Science Garden
Department of Wood Properties and Processing
Tohoku University Botanical Gardens