ForestGEN
Forest EST and Genome database

Description

Cryptomeria japonica (sugi) is an endemic conifer in Japan and is widely grown due to their rapid growth and high wood quality. It belongs to Cupressaceae sensu lato (s.l.). It has 11 pairs of chromosomes, unlike most species in the Pinaceae, which have 12 pairs (or, rarely, 11 or 13) of chromosomes. The estimated C-values is ~10100 Mbp (Hizume et al. 2001); approximately half the mean genome size of Pinaceae species (22470Mbp) (Leitch et al. 2001). C. japonica is a monoecious species, and the allergic reactions caused by its pollen have become a serious public-health problem in Japan.

Yoshihiko Tsumura, Kensuke Yoshimura, Naoki Tani, Tokuko Ujino-Ihara
Forestry and Forest Products Research Institute (FFPRI)
Department of Forest Genetics
Tree Genetics Laboratory

Norihiro Futamura, Kazumasa Yoshida, Kenji Shinohara
Forestry and Forest Products Research Institute (FFPRI)
Department of Molecular and Cell Biology
Plant Molecular Biology Laboratory

Yuzuru Mukai
Gifu Univewrsity

Hiroyuki Kanamori, Hiroko Yamane, Nobukazu Namiki
Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries

Yasushi Totoki, Atsushi Toyoda, Motoaki Seki, Yoshiyuki Sakaki, Kazuo Shinozaki
RIKEN Yokohama Institute

EST : 55543
Cluster : 22116
SubCluster :

Female reproductive organ :	AU299545-AU300308
Inner Bark :	AU066349-AU066493 , AU083584-AU085690 , BP173625-BP176715 , BW992246-BW993916
Inner bark (full length cDNA library) :	DC429779-DC432782
Male reproductive organ :	AU298712-AU299544 , BW993917-BW996365
Male reproductive organ (full-length cDNA library):	BY877900-BY914001
Needle :	DC429774-DC429778
Pollen1 :	BJ936638-BJ940292
Pollen2 :	DC429611-DC429773
Sapwood :	BB940565-BB941614
Seedling :	AU036727-AU036912 , DC429408-DC429610
Vegetative bud :	BW996366-BW997181

# Acknowledgement #

EST project of C. japonica was supported by:
- The Program for Promotion of Basic Research Activities for Innovative Bioscience.
- A Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (grant no. - 15780118).
- Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project grant no. CG1107).
- A Grant-in-Aid Research Project for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries
- A Research Grant from the Forestry and Forest Products Research Institute (no. 200607).
- A Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (grant no. - 18780123).

# References #

1. Tsumura Y., Y. Suyama, K. Yoshimura, N. Shirato, Y. Mukai (1997) Sequence-tagged-sites (STSs) of cDNA clones in Cryptomeria japonica and their evaluation as molecular markers in conifers. Theoretical and Applied Genetics 94:764-772
2. Ujino-Ihara, T., K. Yoshimura, Y. Ugawa, H. Yoshimaru, K. Nagasaka and Y. Tsumura (2000) Expression analysis of ESTs derived from the inner bark of Cryptomeria Japonica Plant Molecular Biology 43: 451-457
3. Ujino-Ihara, T., Y. Taguchi, K. Yoshimura, and Y. Tsumura (2003) Analysis of expressed sequence tags derived from developing seed and pollen cones of Cryptomeria japonica. Plant Biology 5 : 600-607
4. Ujino-Ihara, T., H. Kanamori, H. Yamane, Y. Taguchi, N. Namiki, Y. Mukai, K. Yoshimura, Y. Tsumura (2005) Comparative analysis of expressed sequence tags of conifers and angiosperms reveals sequences specifically conserved in conifers. Plant Molecular Biology 59: 895-907
5. Futamura, N., T.Ujino-Ihara, M. Nishiguchi, H. Kanamori, K. Yoshimura, M. Sakaguchi, K. Shinohara (2006) Analysis of expressed sequence tags from Cryptomeria japonica pollen reveals novel pollen-specific transcripts. Tree Physiology 26: 1517-1528
6. Yoshida, K., M. Nishiguchi, N. Futamura, T. Nanjo (2007) Expressed sequence tags from Cryptomeria japonica sapwood during the drying process. Tree Physiology 27: 1-9
7. Futamura, N., Y. Totoki, A. Toyoda, T. Igasaki, T. Nanjo, M. Seki, Y. Sakaki, A. Mari, K. Shinozaki, K. Shinohara (2008) Characterization of expressed sequence tags from a full-length enriched cDNA library of Cryptomeria japonica male strobili. BMC Genomics 9: 383

# Link #

http://ss.ffpri.affrc.go.jp/labs/cjgenome/indexj.html
(Link to Comparative table for Unigenes.)