ForestGEN
Forest EST and Genome database

Description

Cryptomeria japonica (sugi) is an endemic conifer in Japan and is widely grown due to their rapid growth and high wood quality. It belongs to Cupressaceae sensu lato (s.l.). It has 11 pairs of chromosomes, unlike most species in the Pinaceae, which have 12 pairs (or, rarely, 11 or 13) of chromosomes. The estimated C-values is ~10100 Mbp (Hizume et al. 2001); approximately half the mean genome size of Pinaceae species (22470Mbp) (Leitch et al. 2001). C. japonica is a monoecious species, and the allergic reactions caused by its pollen have become a serious public-health problem in Japan.
In this version, reference transcriptome sequences were constructed focusing on male-sterility gene (MS1)

#Transcripts: 49795

Working ID	Accession	Tissue&
S1s	Nakakubiki-4	MF
S2s	T1NK4F1	MF
S3s	T5_normalMIX	MF
S4s	T5_sterileMIX	MF
S5s	S3T67_normalMIX	MF
S6s	S3T67_sterileMIX	MF
Ooi-7	Ooi-7	IBL
S1NK4	Shindai-1 × Nakabuki-4	MF&IBL
S5HK7	Shindai-5 × Higashikanbara-7	MF&IBL
S8HK5	Shindai-8 × Higashikanbara-5	IBL

&: MF: Male flower; IBL: Inner bark and leaf

# Acknowledgements #

The authors would like to thank Shinji Itoo for producing a part of the materials used in the current study. The Arboretum and Nursery Office (FFPRI) was involved in the maintenance of the C. japonica nursery. Computations were mostly performed on a supercomputer at AFFRIT, MAFF, Japan. This work was partly supported by a Grant-in-Aid from the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (No.28013B), JSPS KAKENHI (Grant Number 25450223), Research grants #201119 and #201421 from the Forestry and Forest Products Research Institute, and grants from the Project of the NARO Bio-oriented Technology Research Advancement Institution (Research program on development of innovative technology (No.28013BC)).

# Data availability #

Sequences and assembled transcripts presented in the current study have been deposited at DDBJ/EMBL/GenBank with accession numbers DRR174638-DRR174656 and ICQT01000001-ICQT01048643, respectively.

# References #

1. Wei, FJ., S. Ueno, T. Ujino-Ihara, M. Saito, Y. Tsumura, Y. Higuchi, S. Hirayama, J. Iwai, T. Hakamata, and Y. Moriguchi, Inspecting abundantly expressed genes in male strobili in sugi (Cryptomeria japonica D. Don) via a highly accurate cDNA assembly. bioRxiv, 2020:2020.04.21.054320.
2. Wei, FJ., S. Ueno, T. Ujino-Ihara, M. Saito, Y. Tsumura, Y. Higuchi, S. Hirayama, J. Iwai, T. Hakamata, and Y. Moriguchi (2021) Construction of a reference transcriptome for the analysis of male sterility in sugi (Cryptomeria japonica D. Don) focusing on MALE STERILITY 1 (MS1). PLoS ONE 16(2): e0247180

# Link #

https://www.ffpri.affrc.go.jp/labs/cjgenome/download.html
(Link to bulk data download)